We constructed a fluorescent sensor of adenylate nucleotides by combining a circularly permuted variant of GFP with a bacterial regulatory protein, GlnK1, from Methanococcus jannaschii. The sensor's ...
Researchers used a novel phosphate-activation reagent, 2-MeImIm-Cl, in an improved coupling reaction to increase the yield of ADP- or ATP-containing molecules. Unlike traditional reactions, the ...
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