In this study, we show that human DNA ligase IV is able to use NAD + as an alternative co-factor for DNA ends ligation. NAD + provides AMP moiety for the adenylation of Ligase IV, which …

Here, we report that human DNA ligase IV, a key enzyme in DNA double-strand break (DSB) repair, is able to use NAD+ as a substrate for double-stranded DNA ligation.

Nov 28, 2018 · In this study, we show that human DNA ligase IV is able to use NAD + as an alternative co-factor for DNA ends ligation. NAD + provides AMP moiety for the adenylation of …

Feb 20, 2019 · All the eukaryotic DNA ligases are known to use adenosine triphosphate (ATP) for DNA ligation. Here, we report that human DNA ligase IV, a key enzyme in DNA double-strand …

Here, we report that human DNA ligase IV, a key enzyme in DNA double-strand break (DSB) repair, is able to use NAD+ as a substrate for double-stranded DNA ligation.

Since this unexpected finding has significant implications for our understanding of the mechanisms and regulation of DNA double strand break repair, we attempted to confirm that …

Here, we report that human DNA ligase IV, a key enzyme in DNA double-strand break (DSB) repair, is able to use NAD+ as a substrate for double-stranded DNA ligation.

Dec 16, 2020 · Here, we provide evidence that NAD+ does not enhance ligation by pre-adenylated DNA ligase IV, indicating that this co-factor is not utilized for re-adenylation and …

Dec 12, 2020 · Moreover, we find that ligation by de-adenylated DNA ligase IV is dependent upon ATP not NAD + or ADP-ribose. Thus, we conclude that human DNA ligase IV cannot use …

DNA ligase is a type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in …