How do you calculate concentration from absorbance?
How do you calculate concentration from absorbance?
Jul 4, 2019
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SOLVED: Detection limit. In spectrophotometry, we measure the concentration of analyte by its absorbance of light. A low concentration sample was prepared, and nine replicate measurements gave absorbances of 0.0047, 0.0054, 0.0062, 0.0060, 0.0046, 0.0056, 0.0052, 0.0044, and 0.0058 in a 1.000-cm cell. Nine reagent blanks gave values of 0.0006, 0.0012, 0.0022, 0.0005, 0.0016, 0.0008, 0.0017, 0.0010, and 0.0011.(a) Find the absorbance detection limit with Equation 5-2.(b) The calibration curve is
SOLVED: Detection limit. In spectrophotometry, we measure t…
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